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- Title
Bioprospecting hot spring metagenome: lipase for the production of biodiesel.
- Authors
Sahoo, Rajesh; Kumar, Mohit; Sukla, Lala; Subudhi, Enketeswara
- Abstract
Screening of metagenomic library from Taptapani Hot Spring (Odisha) yielded a positive lipase clone (pUC-lip479). Sequence analysis showed an ORF (RK-lip479) of 416 amino acid residues which was overexpressed in Escherichia coli BL21 (DE3). Optimum pH and temperature of purified lipase RK-lip479 were 8.0 and 65 °C, respectively, and found to be stable over a pH range of 7.0-9.0 and temperatures 55-75 °C. RK-lip479 could hydrolyse a wide range of 4-nitrophenyl esters (4-nitrophenyoctanoate, 4-nitrophenyldodecanoate, 4-nitrophenylpalmitate, 4-nitrophenylmyristate and 4-nitrophenylstearate), and maximum activity was observed with 4-nitrophenyldodecanoate. RK-lip479 was resistant to many organic solvents, especially isopropanol, DMSO, methanol, DMF, ethanol, dichloromethane, acetone, glycerol and ethyl acetate. RK-lip479 also showed activity in the presence of monovalent (Na and K), divalent (Mg, Mn, Ca, Hg, Cu, Co, Zn and Ag ) and trivalent cations (Fe and Al). Yield of biodiesel production was in the range of 40-76% using various waste oils with RK-Lip479 under optimized conditions.
- Subjects
BIOPROSPECTING; LIPASES; BIODIESEL fuels; ESCHERICHIA coli; NITROPHENYL compounds; ACETONE; GLYCERIN
- Publication
Environmental Science & Pollution Research, 2017, Vol 24, Issue 4, p3802
- ISSN
0944-1344
- Publication type
Article
- DOI
10.1007/s11356-016-8118-7