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- Title
Atomic accuracy in predicting and designing noncanonical RNA structure.
- Authors
Das, Rhiju; Karanicolas, John; Baker, David
- Abstract
We present fragment assembly of RNA with full-atom refinement (FARFAR), a Rosetta framework for predicting and designing noncanonical motifs that define RNA tertiary structure. In a test set of thirty-two 6–20-nucleotide motifs, FARFAR recapitulated 50% of the experimental structures at near-atomic accuracy. Sequence redesign calculations recovered native bases at 65% of residues engaged in noncanonical interactions, and we experimentally validated mutations predicted to stabilize a signal recognition particle domain.
- Subjects
BIOLOGICAL research; EXPERIMENTAL biology; RNA; FERROMAGNETIC materials; MAGNETIC domain; ATOMIC absorption spectroscopy
- Publication
Nature Methods, 2010, Vol 7, Issue 4, p291
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1433