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- Title
The role of Zur-regulated lipoprotein A in bacterial morphology, antimicrobial susceptibility, and production of outer membrane vesicles in Acinetobacter baumannii.
- Authors
Kim, Nayeong; Kim, Hyo Jeong; Oh, Man Hwan; Kim, Se Yeon; Kim, Mi Hyun; Son, Joo Hee; Kim, Seung Il; Shin, Minsang; Lee, Yoo Chul; Lee, Je Chul
- Abstract
Background: Zinc uptake-regulator (Zur)-regulated lipoprotein A (ZrlA) plays a role in bacterial fitness and overcoming antimicrobial exposure in Acinetobacter baumannii. This study further characterized the zrlA gene and its encoded protein and investigated the roles of the zrlA gene in bacterial morphology, antimicrobial susceptibility, and production of outer membrane vesicles (OMVs) in A. baumannii ATCC 17978. Results: In silico and polymerase chain reaction analyses showed that the zrlA gene was conserved among A. baumannii strains with 97–100% sequence homology. Recombinant ZrlA protein exhibited a specific enzymatic activity of D-alanine-D-alanine carboxypeptidase. Wild-type A. baumannii exhibited more morphological heterogeneity than a ΔzrlA mutant strain during stationary phase. The ΔzrlA mutant strain was more susceptible to gentamicin than the wild-type strain. Sizes and protein profiles of OMVs were similar between the wild-type and ΔzrlA mutant strains, but the ΔzrlA mutant strain produced 9.7 times more OMV particles than the wild-type strain. OMVs from the ΔzrlA mutant were more cytotoxic in cultured epithelial cells than OMVs from the wild-type strain. Conclusions: The present study demonstrated that A. baumannii ZrlA contributes to bacterial morphogenesis and antimicrobial resistance, but its deletion increases OMV production and OMV-mediated host cell cytotoxicity.
- Subjects
LIPOPROTEIN A; ACINETOBACTER baumannii; MORPHOLOGY; RECOMBINANT proteins; POLYMERASE chain reaction
- Publication
BMC Microbiology, 2021, Vol 21, Issue 1, p1
- ISSN
1471-2180
- Publication type
Article
- DOI
10.1186/s12866-020-02083-0