We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Anti-tumoral effects of a trypsin inhibitor derived from buckwheat in vitro and in vivo.
- Authors
CHONG-ZHI BAI; MA-LI FENG; XU-LIANG HAO; ZHI-JUAN ZHAO; YU-YING LI; ZHUAN-HUA WANG
- Abstract
Native buckwheat, a common component of food products and medicine, has been observed to inhibit cancer cell proliferation in vitro. The aim of the present study was to evaluate the in vitro and in vivo anti-tumoral effects of recombinant buckwheat trypsin inhibitor (rBTI) on hepatic cancer cells and the mechanism of apoptosis involved. Apoptosis in the H22 cell line induced by rBTI was identified using MTT assays, DNA electrophoresis, flow cytometry, morphological observation of the nuclei, measurement of cytochrome C and assessment of caspase activation. It was identified that rBTI decreases cell viability by inducing apoptosis, as evidenced by the formation of apoptotic bodies and DNA fragmentation. rBTI-induced apoptosis occurred in association with mitochondrial dysfunction, leading to the release of cytochrome C from the mitochondria to the cytosol, as well as the activation of caspase-3, -8 and -9. In conclusion, the results of the present study suggested that rBTI specifically inhibited the growth of the H22 hepatic carcinoma cell line in vitro and in vivo in a concentration-dependent and time-dependent manner, while there were minimal effects on the 7702 normal liver cell line. In addition, rBTI-induced apoptosis in H22 cells was, at least in part, mediated by a mitochondrial pathway via caspase-9.
- Subjects
BUCKWHEAT; TRYPSIN inhibitors; FLOW cytometry; APOPTOSIS; ELECTROPHORESIS; ELECTROCHEMISTRY; LIVER cells
- Publication
Molecular Medicine Reports, 2015, Vol 12, Issue 2, p177
- ISSN
1791-2997
- Publication type
Article
- DOI
10.3892/mmr.2015.3649