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- Title
Large scale plant production of edible bamboo Dendrocalamus asper through somatic embryogenesis.
- Authors
Arya, Sarita; Satsangi, R.; Arya, I. D.
- Abstract
Dendrocalamus asper, an edible bamboo, has a high international trade potential. Somatic embryos and embryogenic callus are induced in D. asper and are used for large-scale propagation. In bamboos somatic embryogenesis has great importance as it resets the internal calendar of plants produced which can be relied upon to live full life span. Embryogenic callus was developed from explants excised from nodal tissues and basal part of leaves that were harvested from in vitro shoots. Somatic embryos were induced on Murashige and Skoog's (1962) medium supplemented with 30µM 2,4-D and 2% (w/v) sucrose in dark conditions. On MS medium supplemented with 9µM 2,4-D + 2.85µM IAA + 0.88µM BAP callus proliferated to 3-5 folds every 4 weeks of subculture with efficiency of 21.7-32.8 globular somatic embryos per embryogenic callus. 6% (w/v) sucrose or 5µM ABA enriched medium enhances maturation of globular somatic embryos to scutellar and coleoptillar somatic embryos within 8 weeks. Somatic embryos developed into plantlets within 30 days on MS medium supplemented with 4.4µM BAP and 2.8µM GA3 with a conversion rate of 70%. Thirty percent of embryos formed only shoots on germination medium. These somatic embryo shoots were multiplied on MS + 13.2µM BAP for 4-5 times of subculture. Later these shoots rooted on 16µM NAA supplemented MS medium with 98% root induction. The plantlets recovered from germination of somatic embryos and micropropagation of somatic embryo derived shoots were successfully hardened, acclimatized and transplanted to the field. The multiplication of D. asper through somatic embryogenesis is now routine procedure without the need for fresh explants. The system of somatic embryogenesis was efficiently utilized for direct plant formation and through in vitro shoot development; more than 10,000 plants have been produced daring last year.
- Subjects
BAMBOO; SOMATIC embryogenesis; BAMBOO shoots; PLANT propagation; CALLUS (Botany)
- Publication
Bamboo Science & Culture, 2008, Vol 21, Issue 1, p21
- ISSN
1535-7635
- Publication type
Article