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- Title
( R)-PAC Biosynthesis in [BMIM][PF]/Aqueous Biphasic System Using Saccharomyces cerevisiae BY4741 Cells.
- Authors
Kandar, Smita; Suresh, A.; Noronha, Santosh
- Abstract
( R)-phenylacetylcarbinol or ( R)-PAC is a pharmaceutical precursor of (1 R, 2 S) ephedrine and (1 S, 2 S) pseudoephedrine. Biotransformation of benzaldehyde and glucose by pyruvate decarboxylase produces ( R)-PAC. This biotransformation suffers from toxicity of the substrate, product [( R)-PAC] and by-product (benzyl alcohol). In the present study, ionic liquid/aqueous biphasic system was employed to enhance ( R)-PAC production. Fermented broth was the reaction medium in which Saccharomyces cerevisiae BY4741 was the source of pyruvate decarboxylase. Hydrophobic ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM][PF]) was the non-aqueous phase in which toxic compounds reside. Biocompatibility of [BMIM][PF] and adequate distribution coefficients of benzaldehyde, ( R)-PAC and benzyl alcohol were determined. A Box-Behnken design and response surface methodology were used for the optimization of biotransformation variables in order to maximize ( R)-PAC yield and productivity. The results showed higher ( R)-PAC yield and productivity of ∼1.5-fold each in the biphasic biotransformation of phase volume ratio 0.05 as compared to the monophasic (conventional) biotransformation. Moreover, the level of major by-product benzyl alcohol was also 3.5-fold lower in biphasic biotransformation. [BMIM][PF]/aqueous biphasic system is a new approach which could intensify the ( R)-PAC production.
- Subjects
METHANOL; CHEMICAL synthesis; PHOSPHATE derivatives; IMIDAZOLES; PHENYL compound derivatives; SACCHAROMYCES cerevisiae; AQUEOUS solutions; PYRUVATE decarboxylase; ACETYL compounds
- Publication
Applied Biochemistry & Biotechnology, 2015, Vol 175, Issue 4, p1771
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-014-1394-0