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- Title
Conventional IVF is feasible in preimplantation genetic testing for aneuploidy.
- Authors
Zhang, Shuoping; Xie, Pingyuan; Lan, Fang; Yao, Yaxin; Ma, Shujuan; Hu, Liang; Tan, Yueqiu; Jiang, Bo; Wan, Anqi; Zhao, Dunmei; Gong, Fei; Lu, Sijia; Lin, Ge
- Abstract
Purpose: To investigate the feasibility of the application of conventional in vitro fertilization (cIVF) for couples undergoing preimplantation genetic testing for aneuploidies (PGT-A) with non-male factor infertility. Methods: To evaluate the efficiency of sperm whole-genome amplification (WGA), spermatozoa were subjected to three WGA protocols: Picoplex, ChromInst, and multiple displacement amplification (MDA). In the clinical studies, 641 couples who underwent PGT-A treatment for frozen embryos between January 2016 and December 2021 were included to retrospectively compare the chromosomal and clinical outcomes of cIVF and intracytoplasmic sperm injection (ICSI). Twenty-six couples were prospectively recruited for cIVF and PGT-A treatment between April 2021 and April 2022; parental contamination was analyzed in biopsied samples; and 12 aneuploid embryos were donated to validate the PGT-A results. Results: Sperm DNA failed to amplify under Picoplex and ChromInst conditions but could be amplified using MDA. In frozen PGT-A cycles, no significant differences in the average rates of euploid, mosaic, and aneuploid embryos per cycle between the cIVF-PGT-A and ICSI-PGT-A groups were observed. The results of the prospective study that recruited couples for cIVF-PGT-A treatment showed no paternal contamination and one case of maternal contamination in 150 biopsied trophectoderm samples. Among the 12 donated embryos with whole-chromosome aneuploidy, 11 (91.7%) presented uniform chromosomal aberrations, which were in agreement with the original biopsy results. Conclusions: Under the Picoplex and ChromInst WGA protocols, the risk of parental contamination in the cIVF-PGT-A cycles was low. Therefore, applying cIVF to couples with non-male factor infertility who are undergoing PGT-A is feasible.
- Subjects
GENETIC testing; INTRACYTOPLASMIC sperm injection; FROZEN human embryos; ANEUPLOIDY; FERTILIZATION in vitro; CHROMOSOME abnormalities
- Publication
Journal of Assisted Reproduction & Genetics, 2023, Vol 40, Issue 10, p2333
- ISSN
1058-0468
- Publication type
Article
- DOI
10.1007/s10815-023-02916-7