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- Title
NIR‐II Excitable Conjugated Polymer Dots with Bright NIR‐I Emission for Deep In Vivo Two‐Photon Brain Imaging Through Intact Skull.
- Authors
Wang, Shaowei; Liu, Jie; Feng, Guangxue; Ng, Lai Guan; Liu, Bin
- Abstract
Methods for noninvasive brain imaging are highly desirable to study brain structures in neuroscience. Two‐photon fluorescence microscopy (2PFM) with near‐infrared (NIR) light excitation is a relatively noninvasive approach commonly used to study brain with high spatial resolution and large imaging depth. However, most of the current studies require cranial window implantation or skull‐thinning methods due to attenuation of excitation light. 2PFM through intact mouse skull is challenging due to strong scattering induced by skull bone. Herein, NIR‐II light excitable single‐chain conjugated polymer dots (CPdots) with bright fluorescence in NIR‐I region (peak at ≈725 nm and quantum yield of 20.6 ± 1.0%) are developed for deep in vivo two‐photon fluorescence (2PF) imaging of intact mouse brain. The synthesized CPdots exhibit good biocompatibility, high photostability, and large two‐photon absorption cross section. The CPdots allow 2PF images acquired upon excitation at 800, 1040 and 1200 nm with the highest signal‐to‐background ratio of 208 demonstrated for 1200 nm excitation. Moreover, a 3D reconstruction of the brain blood vessel network is obtained with a large vertical depth of 400 µm through intact skull. This work demonstrates great potential of bright NIR fluorophores for in vivo deep tissue imaging. NIR‐II light (1200 nm) excitable single‐chain conjugated polymer dots with bright NIR‐I emission (peak at ≈725 nm and quantum yield of 20.6%) is developed for deep in vivo two‐photon imaging of intact mouse brain. High contrast images clearly reveal brain vasculature structure through intact skull with a large depth of 400 µm.
- Subjects
CONJUGATED polymers; BRAIN imaging; SKULL; ABSORPTION cross sections; FLUORESCENCE microscopy; ATTENUATION of light
- Publication
Advanced Functional Materials, 2019, Vol 29, Issue 15, pN.PAG
- ISSN
1616-301X
- Publication type
Article
- DOI
10.1002/adfm.201808365