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- Title
Role of undecan-2-one on ethanol-induced apoptosis in HepG2 cells.
- Authors
F. Castaneda; D. Zimmermann; J. Nolte; J. Baumbach
- Abstract
Abstract  Based on the reduced expression of ethanol-oxidizing enzymes in human hepatocellular carcinoma (HepG2) cells, we analyzed the role of nonoxidative metabolites in ethanol-induced apoptosis in HepG2 cells. For this purpose, an analysis of volatile metabolites of ethanol using ion-mobility spectrometry and gas chromatographyâmass spectrometry was performed. HepG2 cells exposed to 1 mmol/L ethanol exhibited significant synthesis of undecan-2-one compared to untreated cells. Undecan-2-one is a fatty acid ethyl ester metabolite synthesized through a nonoxidative pathway. Undecan-2-one had a dose-dependent cytotoxic effect on HepG2 cells as shown by release of lactate dehydrogenase (LDH). The most notable finding of this study was the potentiation of ethanol-induced apoptosis demonstrated by an increased apoptotic rate induced by undecan-2-one in ethanol-treated HepG2 cells. The data presented in this study contribute to the better understanding of the molecular mechanisms of ethanol exposure at low concentration in HepG2 cells, a human hepatocellular carcinoma-derived cell line.
- Subjects
LIVER cancer; APOPTOSIS; METABOLITES; CELL lines
- Publication
Cell Biology & Toxicology, 2007, Vol 23, Issue 6, p477
- ISSN
0742-2091
- Publication type
Article
- DOI
10.1007/s10565-007-9009-y