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- Title
Effect of Ca<sup>2+</sup> on programmed death of guard and epidermal cells of pea leaves.
- Authors
Kiselevsky, D. B.; Kuznetsova, Yu. E.; Vasil'ev, L. A.; Lobysheva, N. V.; Zinovkin, R. A.; Nesov, A. V.; Shestak, A. A.; Samuilov, V. D.
- Abstract
The effect of Ca2+ on programmed death of guard cells (GC) and epidermal cells (EC) determined from destruction of the cell nucleus was investigated in epidermis of pea leaves. Ca2+ at concentrations of 1–100 μM increased and at a concentration of 1 mM prevented the CN—induced destruction of the nucleus in GC, disrupting the permeability barrier of GC plasma membrane for propidium iodide (PI). Ca2+ at concentrations of 0.1–1 mM enhanced drastically the number of EC nuclei stained by PI in epidermis treated with chitosan, an inducer of programmed cell death. The internucleosomal DNA fragmentation caused by CN− was suppressed by 2 mM Ca2+ on 6 h incubation, but fragmentation was stimulated on more prolonged treatment (16 h). Presumably, the disruption of the permeability barrier of plasma membrane for PI is not a sign of necrosis in plant cells. Quinacrine and diphenylene iodonium at 50 μM concentration prevented GC death induced by CN− or CN− + 0.1 mM Ca2+ but had no influence on respiration and photosynthetic O2 evolution in pea leaf slices. The generation of reactive oxygen species determined from 2′,7′-dichlorofluorescein fluorescence was promoted by Ca2+ in epidermal peels from pea leaves.
- Subjects
PEAS; OSMOSIS; EPIDERMIS; EPITHELIUM; CELL nuclei
- Publication
Biochemistry (00062979), 2010, Vol 75, Issue 5, p614
- ISSN
0006-2979
- Publication type
Article
- DOI
10.1134/S0006297910050111