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- Title
Identification and Characterization of Novel Intracellular a-Xylosidase in Aspergillus oryzae.
- Authors
Tomohiko Matsuzawa; Yusuke Nakamichi; Naoki Shimada
- Abstract
α-Xylosidase releases xylopyranosyl side chains from xyloglucan oligosaccharides and is vital for xyloglucan degradation. Previously, we identified and characterized two α-xylosidases, intracellular AxyA and extracellular AxyB, in Aspergillus oryzae. In this study, we identified a third α-xylosidase, termed AxyC, in A. oryzae. These three A. oryzae α-xylosidases belong to the glycoside hydrolase family 31, but there are clear differences in substrate specificity. Both AxyA and AxyB showed much higher hydrolytic activity toward isoprimeverose (α-D-xylopyranosyl-1,6-glucose) than p-nitrophenyl α-D-xylopyranoside. In contrast, the specific activity of AxyC toward the p-nitrophenyl substrate was approximately 950-fold higher than that toward isoprimeverose. Our study revealed that there are multiple α-xylosidases with different substrate specificities in A. oryzae.
- Subjects
KOJI; ENDOENZYMES; XYLOGLUCANS; GLYCOSIDASES; HYDROLASES
- Publication
Journal of Applied Glycoscience, 2023, Vol 70, Issue 4, p119
- ISSN
1344-7882
- Publication type
Article
- DOI
10.5458/jag.jag.JAG-2023_0007