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- Title
轮叶党参多糖的分离纯化及结构研究.
- Authors
刘艳秋; 王亚茹; 毛迪锐; 常凯
- Abstract
Objective: The structure ol CLPS1 (polysaccharide Iraction 1 ol Codonopsis laceolata) was determined to provide a basis lor the study ol polysaccharide activity ol C. lanceolate and the development ol natural plant polysaccharides. Methods: Alter extraction» separation > and purification > the separation component CLPS1 was determined by gas chromatographyt infrared spectroscopy, nuclear magnetic resonance, periodate oxidation, Smith degradation, and methylation analysis to determine the structure ol CLPS1. Results: The specific rotation ol CLPS1 is +44°, the content ol the total polysaccharide is 97.6%, the uronic acid ol CLPS1 is 13.18%, and the molecular weight ol CLPS1 is 91. 7, The monosaccharide component ol CLPS1 contains Ara, GaU Glc, Gal A, The molar ratio is 2,7: 3.4 : 2.3 : 1. 1; 0.5. Chemical composition and structure ol polysaccharide CLPS1 was studied by inlrared. spectrum, NMR, partial hydrolysis with acid degradation, Smith, methylation and GC-MS. The results showed that monosaccharide compositions ol CLPS1 contained GluA and pyranose> while pyranose included both a-pyranoside and -pyranoside in structure. The backbone was composed ol Ara and Gal, the brancd with Gal and the terminal residues ol backbone with Glu. Branch point ol Gal residues is linked by Glc (If 4,6); Gal (If 3,6); Gal contains If, If 3, If 4,1 f 6,1 f 3, b 乡 Gal contains If, 1~3, 1~4, If 6, If 3, 6; Glc contains If,If 4,If 4,6乡 Ara contains If 5; Galacturonic acid 3. Conclusion: CLPS1 is a complex acidic polysaccharide. The structure analysis ol C. lanceolata polysaccharides is the basis for studying the strueture-activity relationship ol C. lanceolata polysaccharides t which is ol great significance lor understanding the biological activity and mechanism ol C. lanceolata polysaccharides.
- Subjects
NUCLEAR magnetic resonance; GALACTURONIC acid; URONIC acids; INFRARED spectroscopy; MONOSACCHARIDES; CHEMICAL structure; POLYSACCHARIDES
- Publication
Food & Machinery, 2023, Issue 9, p162
- ISSN
1003-5788
- Publication type
Article
- DOI
10.13652/j.spjx.1003.5788.2023.80573