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- Title
Visualizing priming of virus-specific CD8<sup>+</sup> T cells by infected dendritic cells in vivo.
- Authors
Norbury, Christopher C.; Malide, Daniela; Gibbs, James S.; Bennink, Jack R.; Yewdell, Jonathan W.
- Abstract
The rational design of vaccines that elicit CD8+ T cell responses requires knowledge of the identity of the antigen-presenting cell (APC), the location and time of presentation and the nature of the antigen presented by the APC. Here we address these questions for an antigen encoded by a recombinant vaccinia virus. We found that, following local infection, vaccinia virus infected macrophages and dendritic cells in draining lymph nodes. However, only the dendritic cells presented antigen to naïve CD8+ T cells, as determined by direct visualization of sectioned nodes by confocal microscopy. Presentation occurred as rapidly as 6 h after inoculation and quickly declined in parallel with the number of infected cells present in the nodes. These data provide direct evidence that virus-infected APCs prime naïve CD8+ T cells in vivo.
- Subjects
T cells; DENDRITIC cells; VACCINES; IMMUNOLOGY
- Publication
Nature Immunology, 2002, Vol 3, Issue 3, p265
- ISSN
1529-2908
- Publication type
Article
- DOI
10.1038/ni762