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- Title
Comparison of protective and regenerative effects of Hericium erinaceus and NGF in vitro nerve injury.
- Authors
AYHAN, Peray; ÜSTÜN, Ramazan
- Abstract
Peripheral nerve injury (PNI) is a significant health problem that is linked to sensory, motor, and autonomic deficits. Hericium erinaceus (HE), an edible and medicinal mushroom, has been extensively studied for its neurohealth properties. Nerve Growth Factor (NGF) is secreted from Schwann cells in peripheral nerves, endothelial cells, and immune hematopoietic cells. It reduces damaged nerve deaths and increases axon regeneration. In this work, we purposed to compare the neuroprotective and neuritogenic effects of HE and NGF using a laser microdissection technique in a mouse PNI model. We prepared neuron cultures from dorsal root ganglions (DRGs) of 6-8 week aged mice, We created four study groups with the neuron cultures (Axotomy, NGF (50nM), HE (25 μM), NGF(50 nM) +HE (25 μM). We treated them in presence or absence of H. erinaceus and NGF. Axon was cut (axotomy) with a precisely controllable laser beam to model axonal injury in vitro. Axotomized neurons were imaged with fluorescent microscope system. Axotomized neurons's survival ratio and axon length was compared using the fluorescent dyes. Both HE and NGF has protective and regenerative effect on axotomized peripheral sensory neurons. The protective capacity of HE is higher than that of NGF. Furthermore, the together therapy of HE and NGF improved axon regeneration ability of axotomized neurons more than all the other groups. Our results warrant a further investigation of HE and NGF as a potential regenerative agent to treat peripheral nerve injuries.
- Subjects
PERIPHERAL nerve injuries; HERICIUM erinaceus; NERVE growth factor; SCHWANN cells; AXONS
- Publication
Journal of Cellular Neuroscience & Oxidative Stress, 2018, Vol 10, Issue 2, p695
- ISSN
2149-7222
- Publication type
Article