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- Title
Identification of Hypoglycemic Glycolipids from Ipomoea murucoides by Affinity-Directed Fractionation, In Vitro, In Silico and Dynamic Light Scattering Analysis.
- Authors
Rosas-Ramírez, Daniel; Arreguín-Espinosa, Roberto; Escandón-Rivera, Sonia; Andrade-Cetto, Adolfo; Mata-Torres, Gerardo; Pérez-Solís, Ricardo
- Abstract
In the pursuit of identifying the novel resin glycoside modulators glucose-6-phosphatase and α-glucosidase enzymes, associated with blood sugar regulation, methanol-soluble extracts from the flowers of Ipomoea murucoides (cazahuate, Nahuatl), renowned for its abundance of glycolipids, were employed. The methanol-soluble extracts were fractionated by applying the affinity-directed method with glucose-6-phosphatase enzymes from a rat's liver and α-glucosidase enzymes from its intestines. Mass spectrometry and nuclear magnetic resonance were employed to identify the high-affinity compound as a free ligand following the release from the enzymatic complex. Gel permeation through a spin size-exclusion column allowed the separated high-affinity molecules to bind to glucose-6-phosphatase and α-glucosidase enzymes in solution, which led to the identification of some previously reported resin glycosides in the flowers of cazahuate, where a glycolipid mainly structurally related to murucoidin XIV was observed. In vitro studies demonstrated the modulating properties of resin glycosides on the glucose-6-phosphatase enzyme. Dynamic light scattering revealed conformational variations induced by resin glycosides on α-glucosidase enzyme, causing them to become more compact, akin to observations with the positive control, acarbose. These findings suggest that resin glycosides may serve as a potential source for phytotherapeutic agents with antihyperglycemic properties.
- Subjects
GLYCOLIPIDS; LIGHT scattering; LIVER enzymes; IPOMOEA; GLUCOSIDASES; NUCLEAR magnetic resonance; GLUCOSE-6-phosphatase
- Publication
Plants (2223-7747), 2024, Vol 13, Issue 5, p644
- ISSN
2223-7747
- Publication type
Article
- DOI
10.3390/plants13050644