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- Title
AMPK Subunit Expression Regulates Intramuscular Fat Content and Muscle Fiber Type in Chickens.
- Authors
Ye YANG; Jiao SONG; Ruiqi FU; Yanfa SUN; Jie WEN
- Abstract
The objective of this study was to assess the role of AMPK in intramuscular fat (IMF) and fiber type in chicken muscle. The chickens were slaughtered and their muscles were collected at the ages of 4, 8, and 16 weeks so as to determine the IMF contents, as well as the expression levels of AMPK subunits, regulators of adipogenesis. In addition, the myosin heavy chains (MyHCs) in thigh muscle tissues were also measured. The results showed that the IMF contents in 16-week old chickens were higher than those in 4 and 8-week-old chickens (P<0.05). The expression levels of fatty acid synthase (FAS) and fatty aicd translocase CD36 (FAT/CD36) mRNA were increased significantly in samples collected at the ages of 4 and 16 weeks (P<0.05). The expression levels of MyHC IIa and IIb differed significantly among all the developmental stages (P<0.05). The AMPKα2, AMPKγ1, and AMPKγ3 mRNA levels were dramatically decreased with the increase of age (P<0.05). To examine the role of AMPK in adipogenesis regulation, the SV cells were cultured in an adipogenesis medium and treated with AICAR and Compound C respectively, the specific activator and inhibit of AMPK. The Compound C induced dramatically a greater expression of C/EBPβ, SREBP1 and PPARγ (P<0.05). In conclusion, the expression of AMPKα2, AMPKγ1, and AMPKγ3 mRNA is significantly correlated with the adipogenesis in skeletal muscle of chickens.
- Subjects
GENETIC regulation; CHICKENS; ANIMAL genetics; PROTEIN kinase regulation; SKELETAL muscle; ADIPOGENESIS; POULTRY; GENETICS
- Publication
Agricultural Science & Technology, 2015, Vol 16, Issue 5, p1006
- ISSN
1009-4229
- Publication type
Article