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- Title
Immunization with a tetramer derivative of an anti-inflammatory pentapeptide produced byEntamoeba histolyticaprotects gerbils (Meriones unguiculatus) against experimental amoebic abscess of the liver.
- Authors
Giménez-Scherer, Juan Antonio; Cárdenas, Guadalupe; López-Osuna, Martha; Velázquez, Juan Raymundo; Rico, Guadalupe; Isibasi, Armando; Maldonado, Ma. del Carmen; Morales, Ma. Esther; Fernández-Diez, Jorge; Kretschmer, Roberto R.
- Abstract
Axenically grownEntamoeba histolyticaproduces a pentapeptide (Met-Gln-Cys-Asn-Ser) with several anti-inflammatory properties, including the inhibition of human monocyte locomotion (Monocyte Locomotion Inhibitory Factor(MLIF)). A construct displays the same effects as the native material. It remains to be seen if MLIF is used, or even producedin vivoby the tissue-invading parasite. If MLIF were to be relevant in invasive amoebiasis, immunizing against it could diminish this parasite advantage and prevent lesions. KLH-linked MLIF mixed with Freund's adjuvant was too aggressive an immunizing material to answer this question. However, immunization with a tetramer of MLIF (but not ascrambledversion of MLIF) around a lysine core (MLIF–MAPS), that displays increased antigenicity, yet lacks excessive innate immunity activation, completely protects gerbils against amoebic abscess of the liver caused by the intraportal injection of virulentE. histolytica.Liver abscesses caused byListeria monocytogeneswere not prevented. InvasiveE. histolyticamay produce the parent protein of MLIFin vivo, and if appropriately cleaved, it may play a role in invasive amoebiasis. MLIF may join new vaccination strategies against amoebiasis.
- Subjects
ENTAMOEBA histolytica; IMMUNIZATION; ENTAMOEBA; PEPTIDES; LIVER diseases; AMEBIASIS
- Publication
Parasite Immunology, 2004, Vol 26, Issue 8/9, p343
- ISSN
0141-9838
- Publication type
Article
- DOI
10.1111/j.0141-9838.2004.00718.x