We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Prolonged incubation of processed human spermatozoa will increase DNA fragmentation.
- Authors
Nabi, A.; Khalili, M. A.; Halvaei, I.; Roodbari, F.
- Abstract
One of the causes of failure in ART is sperm DNA fragmentation which may be associated with long period of spermatozoa incubation at 37 °C. The objective was to evaluate the rate of sperm DNA fragmentation using the sperm chromatin dispersion ( SCD) test after swim-up at different time intervals prior to use. In this prospective study, 21 normozoospermic specimens were analysed. The samples were incubated at 37 °C after preparation by direct swim-up. DNA fragmentation was assessed at different time intervals (0, 1, 2 and 3 h) using SCD test. Spermatozoa with no DNA fragmentation showed large- or medium-sized halos, and sperm cells with DNA fragmentation showed either a small halo or no halo . The rates of normal morphology and progressive motility after sperm processing were 72.33 ± 2.53% and 90 ± 1.02%, respectively. The rate of sperm DNA fragmentation was significantly higher after 2 h (8.81 ± 0.93%, P = 0.004) and 3 h (10.76 ± 0.89%, P < 0.0001) of incubation compared to 0 h (4.38 ± 0.8%). A positive correlation was found between the incubation time and sperm DNA damage ( P < 0.0001). Prolonged incubation of prepared normozoospermic samples at 37 °C is associated with higher rates of sperm DNA fragmentation. Therefore, sperm samples intended for ART procedures should be used within 2 h of incubation at 37 °C.
- Subjects
SPERM motility; SPERMATOZOA physiology; SPERMATOZOA; DNA damage; CHROMATIN; SEMEN analysis; MALE infertility
- Publication
Andrologia, 2014, Vol 46, Issue 4, p374
- ISSN
0303-4569
- Publication type
Article
- DOI
10.1111/and.12088