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- Title
Nearly complete structure of bacteriophage DT57C reveals architecture of head-to-tail interface and lateral tail fibers.
- Authors
Ayala, Rafael; Moiseenko, Andrey V.; Chen, Ting-Hua; Kulikov, Eugene E.; Golomidova, Alla K.; Orekhov, Philipp S.; Street, Maya A.; Sokolova, Olga S.; Letarov, Andrey V.; Wolf, Matthias
- Abstract
The T5 family of viruses are tailed bacteriophages characterized by a long non-contractile tail. The bacteriophage DT57C is closely related to the paradigmal T5 phage, though it recognizes a different receptor (BtuB) and features highly divergent lateral tail fibers (LTF). Considerable portions of T5-like phages remain structurally uncharacterized. Here, we present the structure of DT57C determined by cryo-EM, and an atomic model of the virus, which was further explored using all-atom molecular dynamics simulations. The structure revealed a unique way of LTF attachment assisted by a dodecameric collar protein LtfC, and an unusual composition of the phage neck constructed of three protein rings. The tape measure protein (TMP) is organized within the tail tube in a three-stranded parallel α-helical coiled coil which makes direct contact with the genomic DNA. The presence of the C-terminal fragment of the TMP that remains within the tail tip suggests that the tail tip complex returns to its original state after DNA ejection. Our results provide a complete atomic structure of a T5-like phage, provide insights into the process of DNA ejection as well as a structural basis for the design of engineered phages and future mechanistic studies. The authors present the nearly-complete structure of the DT57C bacteriophage of the Siphovirus family, revealing the molecular architecture of its capsid, neck, tail and tail tip, and providing insights into the process of DNA ejection.
- Subjects
BACTERIOPHAGES; MOLECULAR dynamics; ATOMIC structure; ATOMIC models; TAPE measures; STRUCTURAL design
- Publication
Nature Communications, 2023, Vol 14, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-023-43824-9