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- Title
Dermatophyte Identification In Skin And Nail Samples Using Molecular Method.
- Authors
Vishnoi, Sakshi; Rajput, Madhurendra Singh
- Abstract
Objective- The microscope investigation of fungi is rapid but these diagnostic procedures show lack of sensitivity and specificity with 15% fallacious results and species or genus level identification is not feasible. Material and Methods- This 18-month prospective study included all dermatophytes that were isolated during that time. Dermatophytes were discovered using standard phenotypic techniques. Therefore, in recent years many molecular methods have been developed which are more specific, precise, rapid and cost effective than conventional phenotypic methods. Samples of skin and nails were analyzed under a microscope using 20% KOH and cultivated on Sabouraud dextrose agar supplemented with DTM Media, cycloheximide, and chloramphenicol. By sequencing the internal transcribed spacer (ITS) r-DNA region, the identity of the fungal isolates was verified. Results- Out Of the 320 samples 270 (84.37%) samples showed growth of fungi (dermatophytes) after culturing on SDA media. Whereas with AP-PCR, 303 (94.68%) samples were positive for dermatophytes. Seven species of dermatophyte namely Trichophyton mentagrophytes, Trichophyton rubrum, Epidermophyton floccosum, Trichophyton tonsurans, Microsporum gypseum, Trichophyton verrucosum and Microsporum canis identified after culturing on SDA media from these 270 samples were confirmed by AP-PCR also.
- Subjects
NAILS (Anatomy); DERMATOPHYTES; MICROSPORUM; FUNGAL growth; TRICHOPHYTON
- Publication
Journal of Cardiovascular Disease Research (Journal of Cardiovascular Disease Research), 2024, Vol 15, Issue 6, p794
- ISSN
0975-3583
- Publication type
Article