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- Title
ENZYMATIC SYNTHESIS OF NUCLEOSIDES.
- Authors
Beresnev, A. I.; Kvach, S. V.; Sivets, G. G.; Zinchenko, A. I.
- Abstract
Using advanced genetic engineering methodology, a series of silent mutations was originally introduced into initial fragment of gene cloned in E. coli and coding for pyrimidine nucleoside phosphorylase of T. thermophilus. As a result, 70% rise in the synthesis level of studied thermostable enzyme was achieved. We were able to establish that pyrimidine fluoro-deoxy nucleosides may serve as substrates of phosphorolysis reaction catalyzed by thermostable pyrimidine nucleoside phosphorylase of T. thermophilus. Moreover, recombinant nucleoside phosphorylases were never before involved in enzymatic synthesis of biologically significant purine 3'-fluoronucleosides
- Subjects
NUCLEOSIDE synthesis; PYRIMIDINE nucleosides; ESCHERICHIA coli; GENETIC engineering; PHOSPHORYLASES; NUCLEOSIDE derivatives; MOLECULAR cloning; PYRIMIDINES
- Publication
Journal of Nature Science & Sustainable Technology, 2022, Vol 16, Issue 4, p275
- ISSN
1933-0324
- Publication type
Article