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- Title
Introgression of blast resistance genes into the elite rice variety MR263 through marker-assisted backcrossing.
- Authors
Hasan, Muhammad M; Rafii, Mohd Y; Ismail, Mohd Razi; Mahmood, Maziah; Alam, Md Amirul; Abdul Rahim, Harun; Malek, Mohammad A; Latif, Mohammad Abdul
- Abstract
BACKGROUND Blast caused by the fungus Magnaporthe oryzae is a significant disease threat to rice across the world and is especially prevalent in Malaysia. An elite, early-maturing, high-yielding Malaysian rice variety, MR263, is susceptible to blast and was used as the recurrent parent in this study. To improve MR263 disease resistance, the Pongsu Seribu 1 rice variety was used as donor of the blast resistance Pi-7(t), Pi-d(t)1 and Pir2-3(t) genes and qLN2 quantitative trait locus ( QTL). The objective was to introgress these blast resistance genes into the background of MR263 using marker-assisted backcrossing with both foreground and background selection. RESULTS Improved MR263-BR-3-2, MR263-BR-4-3, MR263-BR-13-1 and MR263-BR-26-4 lines carrying the Pi-7(t), Pi-d(t)1 and Pir2-3(t) genes and qLN2 QTL were developed using the simple sequence repeat ( SSR) markers RM5961 and RM263 (linked to the blast resistance genes and QTL) for foreground selection and a collection of 65 polymorphic SSR markers for background selection in backcrossed and selfed generations. A background analysis revealed that the highest rate of recurrent parent genome recovery was 96.1% in MR263-BR-4-3 and 94.3% in MR263-BR-3-2. CONCLUSION The addition of blast resistance genes can be used to improve several Malaysian rice varieties to combat this major disease. © 2015 Society of Chemical Industry
- Subjects
MALAYSIA; PYRICULARIA oryzae; RICE blast disease prevention; MOLECULAR evolution; GENETIC regulation; RICE
- Publication
Journal of the Science of Food & Agriculture, 2016, Vol 96, Issue 4, p1297
- ISSN
0022-5142
- Publication type
Article
- DOI
10.1002/jsfa.7222