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- Title
Hypoglycaemic effects of functional tri-peptides from silk in differentiated adipocytes and streptozotocin-induced diabetic mice.
- Authors
Han, Bok Kyung; Lee, Hyun Jung; Lee, Hyun ‐ Sun; Suh, Hyung Joo; Park, Yooheon
- Abstract
BACKGROUND: In this study, the tri-peptides Gly-Glu-Tyr (GEY) and Gly-Tyr-Gly (GYG), identified previously as active compounds from the silk peptide E5K6, significantly stimulated basal and insulin-mediated glucose uptake by 3T3-L1 fibroblasts in a dose-dependent manner. RESULTS: Synthetic GEY and GYG peptides at a concentration of 500 μmol L-1 significantly increased glucose transporter type 4 expression by 157% and 239%, respectively. Differentiation of 3T3-L1 cells into adipocytes leads to accumulation of intracellular fat droplets, and GEY and GYG at a concentration of 250 μmol L-1 suppressed this effect by 72% and 75%, respectively. GYG improved glucose tolerance in steptozotocin (STZ)-induced diabetic mice in a dose-dependent manner. CONCLUSION: These results suggest that GYG isolated from E5K6 has anti-diabetic potential and silkwaste products containing bioactive peptides could be used to the developments of treatments to lower blood glucose.
- Subjects
FAT cells; STREPTOZOTOCIN; CELL differentiation; HYPOGLYCEMIA treatment; BIOACTIVE compounds; LABORATORY mice; THERAPEUTICS
- Publication
Journal of the Science of Food & Agriculture, 2016, Vol 96, Issue 1, p116
- ISSN
0022-5142
- Publication type
Article
- DOI
10.1002/jsfa.7067