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- Title
The Role of Ultraweb Nano-Fibrillar Substrates in the Differentiation of In Vitro Mouse Bone Marrow Mesenchymal Stem Cell-Derived Hepatocyte-Like Cells.
- Authors
Piryaei, Abbas; Valojerdi, Mojtaba Rezazadeh; Shahsavani, Mansoureh; Baharvand, Hossein
- Abstract
Objective: To study the impact of ultraweb nano-fibrillar substrates on in vitro differentiation of mouse bone marrow-derived mesenchymal stem cells into hepatocyte-like cells. Materials and Methods: For a duration of up to 36 days, mouse bone marrow mesenchymal stem cells (MSCs) were cultured on an artificial basement membrane containing ultraweb nanofiber (nano+) and plates (nano-) coated with Matrigel and used growth and differentiating factors for hepatogenic differentiation. Then, to study the impact of nanofibers on hepatocyte differentiation, mRNA expressions for liver-specific genes (CK7, AFP, FOXa2, ALB, CYP1a1, HNF4a) were analyzed using real-time PCR , and ultrastructures of differentiated cells were evaluated using scanning and transmission electron microscope. Results: Both experimental groups expressed mRNA of liver-specific genes in a time dependent manner. However, mRNA expression trends of liver-specific genes, especially those of FOXa2 and HNF4a, were better in the nano culture as compared to the nano culture (p<0.05). Furthermore, the ultrastructures of differentiated cells in the 36 day-old nano culture were more mature as compared to those of the nano group. Conclusion: The results suggest that topographical properties of the extracellular matrices produced by ultraweb nanofibers are effective for the in vitro reproduction of more differentiated MSC-derived hepatocyte-like cells.
- Subjects
CYTOLOGICAL research; NANOFIBERS; BONE marrow; MESENCHYMAL stem cells; ELECTRON microscopes; IMMUNE system
- Publication
Yakhteh Medical Journal, 2010, Vol 12, Issue 2, p275
- ISSN
1561-4921
- Publication type
Article