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- Title
Effect of hydrophilic titanium surface on the proliferation and differentiation of osteoblasts.
- Authors
DU Juan; JIANG Huan-huan; MO Jia-ji; ZHOU Zhong-hao; ZHANG Zhi-yong; LAI Hong-chang
- Abstract
PURPOSE: To evaluate the effect of sand-blasted, large-grit, acid-etched (SLA) and chemically-modified SLA (modSLA/SLActive) titanium surfaces on MC3T3-E1 murine pre-osteoblast cell proliferation and differentiation. METHODS: Hydrophilic SLA (modSLA) surface of titanium disks were chemically-modified; sand-blasted, large-grit, acid-etched (SLA) and smooth pretreated (FT) titanium disks were produced. MTT assay, alkaline phosphatase (ALP) activity and Alizarin red-S (ARS)were used to evaluate MC3T3-E1 pre-osteoblast cell behaviors on different titanium surfaces. Real-time PCR was applied to detect the expression of osteogenic genes including Runx2, OSX, OCN and OPN of the MC3T3-E1 cells cultured on the three groups of substrates. The data was analyzed by using SAS 9.0 software package. RESULTS: ALP activities, calcium deposition and the expression of Runx2, OSX, OPN and OCN were significantly higher and the cell proliferation was significantly lower on SLA surfaces than on PT surfaces. With regard to modSLA surface, the effect was even more significant (P<0.05). CONCLUSIONS: Surface roughness and hydrophilicity are two important factors that determine osteoblasts behaviors. Surface roughness of titanium substrates seems to enhance the osteoblastic differentiation and decrease the proliferation of MC3T3-E1 cells. Osteogenic responses to modSLA were better than to hydrophobic SLA surface. Supported by National Natural Science Foundation of China (31070842), Key Research Project of Science and Technology Commission of Shanghai Municipality (09411955000), and Nano-Science and Technology Project of Science and Technology Commission of Shanghai Municipality (1052nm04500).
- Subjects
OSTEOBLASTS; TITANIUM; CELL proliferation; CELL differentiation; SURFACE roughness; CELL culture; BACTERIA hydrophilicity &; hydrophobicity; NATIONAL Natural Science Foundation of China (Organization)
- Publication
China Journal of Oral & Maxillofacial Surgery, 2012, Vol 10, Issue 3, p182
- ISSN
1672-3244
- Publication type
Article