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- Title
Evaluation of the MYC_ CANFA gene in Chinese Shar Peis with cobalamin deficiency.
- Authors
Grützner, Niels; Bishop, Micah A.; Suchodolski, Jan S.; Steiner, Jörg M.
- Abstract
Background A recent genome-wide scan using the canine minimal screening set 2 ( MSS-2) showed that cobalamin deficiency appears to be hereditary in Chinese Shar Peis and is linked to the microsatellite markers DTR13.6 and REN13N11 on canine chromosome 13. Objective The goal of this study was to evaluate the MYC_ CANFA gene, which is the closest known gene with a distance of approximately 0.06 megabases (Mb) to the microsatellite marker DTR13.6, for any mutations in this breed. Methods Microsatellite markers (Myc and G15987) for genotyping and primers for sequencing were used to evaluate the MYC_ CANFA gene. The genotype and gene sequence were compared between cobalamin-deficient Shar Peis, Shar Peis with normal serum cobalamin concentrations, and the DNA sequences published as part of the Ensemble Genomic map. Results Neither the microsatellite markers (Myc and G15987) nor the sequences of the MYC_ CANFA gene showed a significant difference among both groups of Shar Peis and the published canine DNA sequence. Conclusions The data presented here suggest that cobalamin deficiency in Shar Peis is not related to any mutations of the MYC_ CANFA gene according to the genotyping and sequencing results in this study. Further investigations are warranted to find a potential genomic locus in proximity to DTR13.6 and REN13N11 that shows mutations in cobalamin-deficient Shar Peis.
- Subjects
CHINESE Shar-Pei; VITAMIN B12 deficiency; MYC oncogenes; MICROSATELLITE repeats; NUCLEOTIDE sequence; VETERINARY genetics; VETERINARY medicine
- Publication
Veterinary Clinical Pathology, 2013, Vol 42, Issue 1, p61
- ISSN
0275-6382
- Publication type
Article
- DOI
10.1111/vcp.12010