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- Title
How deep is enough in single-cell RNA-seq?
- Authors
Streets, Aaron M; Huang, Yanyi
- Abstract
The article presents quantitative guidelines for determining the sequencing depth vital to distinguish the cell types in a complex sample. Accurate and reliable classification of cell types had been demonstrated with the use of a commercial microfluidic platform to capture hundreds of cells from a variety of human tissues and performing RNA-seq to different depths. It suggests that a comprehensive understanding of the capabilities and limits of RNA-seq technology is important.
- Subjects
RNA sequencing; RNA analysis; MICROFLUIDICS; MICROFLUIDIC devices; MICROTECHNOLOGY
- Publication
Nature Biotechnology, 2014, Vol 32, Issue 10, p1005
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt.3039