The article focuses on reports of the Sequencing Quality Control(SEQC)/Microarray Quality Control (MAQC)-III Consortium and the Association of Biomolecular Resource Facilities (ABRF) related to result of performance characteristics of RNA-sequencing (RNA-seq) as of September 2014. Topics discussed include importance of results for future research projects, limitations of microarrays and ability of RNA-seq to detect novel splice sites.