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- Title
Estimation of glomerular volume: A comparison of four methods.
- Authors
Lane, Pascale H.; Steffes, Michael W.; Mauer, S. Michael
- Abstract
Method for estimating glomerular volume were compared in Zenker-fixed, paraffin-embedded biopsies from 10 patients with insulin-dependent diabetis mellitus and 6 normal kidney donors. Two methods of measurement of individual glomerular volumes were used: the Cavalieri method (considered the ‘gold standard’) and the maximal profile area (MHA) method. Also studied were the method of Weibel and Gomez and a method based on the disector principle; both estimate mean volume (VG). MPA and Cavalieri showed strong correlation (r = 0.93; P < 0.001), although the MPA method consistently overestimated the true volume; six glomeruli were necessary for a reliable estimate of VG. The disector method did not correlate with VG determined by Cavalieri. WeibelGomez did correlate with Cavalieri (r = 0.68; P <0 0.05), but overestimated VG. At least 15 profiles were needed to provide a dependable estimate of VG, by WeibelGomez. The Cavalieri, MPA, and WeibelGomez, methods all can provide reliable estimates of VG, the latter two with appropriate correction factors. The individual glomerular volume methods, while more time consuming, provide information on variation and distribution of the glomerular population and are the methods of choice for studies of glomerular volume.
- Subjects
KIDNEY glomerulus; DIABETES; COMPARATIVE studies; RESEARCH; CLINICAL pathology; DIAGNOSIS; PATHOLOGY
- Publication
Kidney International, 1992, Vol 41, Issue 4, p1085
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1992.165