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- Title
Application of Fluorescence In Situ Hybridization on Cerebrospinal Fluid Cytospins for the Detection of Residual Leukemic Cells in Patients With Childhood Acute Lymphoblastic Leukemia.
- Authors
Hwang, Sang Mee; Park, Hee Sue; Park, Seungman; Kim, Sung-Min; Hong, Kyung Taek; Chang, Yoon Hwan; Lee, Dong Soon
- Abstract
<bold>Objectives: </bold>Diagnosis of central nervous system involvement in acute lymphoblastic leukemia (ALL) requires morphologic expertise; therefore, we evaluated interphase fluorescence in situ hybridization (iFISH) of cerebrospinal fluid (CSF) cytospin preparations as a potential complementary test.<bold>Methods: </bold>Twenty-three CSF cytospin specimens from 13 pediatric patients with ALL were included. iFISH probes detecting BCR-ABL1, ETV6-RUNX1, and KMT2A rearrangement and CDKN2A deletion, which were present at initial diagnosis, were used on follow-up CSF cytospin specimens and were compared with cytology.<bold>Results: </bold>Seventeen (73.9%) follow-up specimens showed concordant results between iFISH and cytology. Two (8.7%) samples with discordant results were positive by iFISH but not by cytology; one (4.3%) was positive only by cytology. In the remaining three (13.0%) specimens, too few cells were available for cytology, whereas iFISH interpretation was possible.<bold>Conclusions: </bold>iFISH of CSF cytospin preparations improves malignant cell detection in pediatric ALL.
- Subjects
NERVOUS system; ORGANS (Anatomy); HAIRY cell leukemia; LYMPHOBLASTIC leukemia diagnosis; COMPARATIVE studies; CYTOGENETICS; LONGITUDINAL method; LYMPHOBLASTIC leukemia; RESEARCH methodology; MEDICAL cooperation; RESEARCH; FLUORESCENCE in situ hybridization; EVALUATION research
- Publication
American Journal of Clinical Pathology, 2019, Vol 151, Issue 4, p416
- ISSN
0002-9173
- Publication type
journal article
- DOI
10.1093/ajcp/aqy160