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- Title
分子标记辅助选育甘蓝型油菜高油酸pol TCMS不育 两用系及其恢复系.
- Authors
郭聚领; 石笑蕊; 辛强; 洪登峰; 杨光圣
- Abstract
High oleic rapeseed (Brassica napus L.) oil has high nutrition with good quality for cooking, processing and storage. For high oleic acid breeding and heterosis utilization, male sterile line and restoring line were bred by using line J-3111 with high oleic acid as donor parent. Its high oleic allele (fad2 gene) was succefully transferred into a thermo-sensitive pol cytoplasmic male sterile (pol TCMS) line 616A and a restoring line L-135R by back⁃ cross and molecular marker-assisted selection. Results showed that oleic content of the improved strains was up to 75.18%. Meanwhile, yield traits of the improved lines showed no significant difference comparing with recipient parents in silique length, seeds number per silique and thousand-seeds weight. Based on whole-genome resequencing data, InDel markers were developed closely-linked with fad2 gene. Thus we obtained improved pol TCMS line and restoring line with high oleic contents, which laid foundation for breeding of high-yield and high-oleic cotent.
- Subjects
RAPESEED; OLEIC acid; NUTRITION; ALLELES; HETEROSIS; SEEDS; RAPESEED oil
- Publication
Chinese Journal of Oil Crop Sciences, 2021, Vol 43, Issue 3, p418
- ISSN
1007-9084
- Publication type
Article
- DOI
10.19802/j.issn.1007-9084.2020132