We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effectiveness of Six Different Disinfectants on Removing Five Microbial Species and Effects on the Topographic Characteristics of Acrylic Resin.
- Authors
Da Silva, Francine Cristina; Kimpara, Estevão Tomomitsu; Mancini, Maria Nadir Gasparotto; Balducci, Ivan; Jorge, Antonio Olavo Cardoso; Koga-Ito, Cristiane Yumi
- Abstract
Purpose: The aim of this study was to evaluate the effectiveness of disinfectant solutions (1% sodium hypochlorite, 2% chlorhexidine digluconate, 2% glutaraldehyde, 100% vinegar, tabs of sodium perborate-based denture cleanser, and 3.8% sodium perborate) in the disinfection of acrylic resin specimens (n = 10/group) contaminated in vitro by Candida albicans, Streptococcus mutans, S. aureus, Escherichia coli, or Bacillus subtilis as measured by residual colony-forming unit (CFU). In a separate experiment, acrylic resin was treated with disinfectants to monitor potential effects on surface roughness, Ra (μm), which might facilitate microbial adherence. Materials and Methods: Three hundred fifty acrylic resin specimens contaminated in vitro with 1×106 cells/ml suspensions of standard strains of the cited microorganisms were immersed in the disinfectants for 10 minutes; the control group was not submitted to any disinfection process. Final counts of microorganisms per ml were performed by plating method for the evaluation of microbial level reduction. Results were compared statistically by ANOVA and Tukey's test ( p≤ 0.05). In a parallel study aiming to evaluate the effect of the tested disinfectant on resin surface, 60 specimens were analyzed in a digital rugosimeter before and after ten cycles of 10-minute immersion in the disinfectants. Measurements of superficial roughness, Ra (μm), were compared statistically by paired t-test ( p≤ 0.05). Results: The results showed that 1% sodium hypochlorite, 2% glutaraldehyde, and 2% chlorhexidine digluconate were most effective against the analyzed microorganisms, followed by 100% vinegar, 3.8% sodium perborate, and tabs of sodium perborate-based denture cleanser. Superficial roughness of the specimens was higher after disinfection cycles with 3.8% sodium perborate ( p= 0.03) and lower after the cycles with 2% chlorhexidine digluconate ( p= 0.04). Conclusion: Within the limits of this experiment, it could be concluded that 1% sodium hypochlorite, 2% glutaraldehyde, 2% chlorexidine, 100% vinegar, and 3.8% sodium perborate are valid alternatives for the disinfection of acrylic resin.
- Subjects
DISINFECTION &; disinfectants; ACRYLIC resins; DENTURES; INFECTION treatment; DENTISTRY; SURFACE roughness
- Publication
Journal of Prosthodontics, 2008, Vol 17, Issue 8, p627
- ISSN
1059-941X
- Publication type
Article
- DOI
10.1111/j.1532-849X.2008.00358.x