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- Title
Kinetics of Azanone (HNO) Reactions with Thiols: Effect of pH.
- Authors
Smulik-Izydorczyk, Renata; Dębowska, Karolina; Rostkowski, Michał; Adamus, Jan; Michalski, Radosław; Sikora, Adam
- Abstract
HNO (nitroxyl, IUPAC name azanone) is an electrophilic reactive nitrogen species of growing pharmacological and biological significance. Here, we present data on the pH-dependent kinetics of azanone reactions with the low molecular thiols glutathione and N-acetylcysteine, as well as with important serum proteins: bovine serum albumin and human serum albumin. The competition kinetics method used is based on two parallel HNO reactions: with RSH/RS− or with O2. The results provide evidence that the reaction of azanone with the anionic form of thiols (RS−) is favored over reactions with the protonated form (RSH). The data are supported with quantum mechanical calculations. A comprehensive discussion of the HNO reaction with thiolates is provided.
- Subjects
REACTIVE nitrogen species; PH effect; THIOLS; BLOOD proteins; NITROXYL; SERUM albumin
- Publication
Cell Biochemistry & Biophysics, 2021, Vol 79, Issue 4, p845
- ISSN
1085-9195
- Publication type
Article
- DOI
10.1007/s12013-021-00986-x