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- Title
Ultra‐high‐performance liquid chromatography‐Quadrupole‐Exactive‐Orbitrap‐tandem mass spectrometry‐based putative identification for Eucommiae Folium (Duzhongye) and its quality‐marker candidate for pharmacopeia
- Authors
Liu, Shuqin; Li, Xican; Cai, Rongxin; Chen, Ban; Zeng, Jingyuan; Li, Chunhou; Zhou, Xianxi; Li, Yueying
- Abstract
Eucommiae Folium (Duzhongye) is a traditional Chinese medicine with a long history of use in China. However, its quality‐marker in Chinese Pharmacopoeia is poorly defined nowadays. The study, therefore, conducted an ultra‐high‐performance liquid chromatography coupled with hybrid quadrupole‐orbitrap tandem mass spectrometry analysis to obtain accurate data. The obtained data were then compared with the authentic standards library using Xcalibur 4.1 software package and TraceFinder General Quan. Through the comparison, the study has putatively identified 26 bioactive compounds, which include 17 flavonoid derivatives (catechin, quercetin 3‐gentiobioside, quercetin 3‐O‐β‐D‐glucose‐7‐O‐β‐D‐gentiobioside, taxifolin, myricetin 3‐O‐galactoside, myricitrin, hyperoside, rutin, isoquercitrin, quercetin 3‐O‐β‐xylopyranoside, quercitrin, isorhamnetin 3‐O‐β‐D‐glucoside, quercetin, kaempferol, S‐eriodictyol, S‐naringenin, and phloridzin), four caffeoylquinic acids (neochlorogenic acid, chlorogenic acid, isochlorogenic acid A, and isochlorogenic acid C), two alkaloids (vincamine and jervine), one lignan (pinoresinol), one xanthone (cowaxanthone B), and one steroid (cholesteryl acetate). Of these, flavonoid isoquercitrin is recommended as the new and additional pharmacopeia quality‐marker candidate, which can not only overcome the unreliability of old quality‐marker but also recognize the possible counterfeit.
- Subjects
CHINA; FLAVONOLS; TANDEM mass spectrometry; MANGIFERIN; XANTHONE; CHLOROGENIC acid; CHINESE medicine; BIOACTIVE compounds; FLAVONOIDS; QUERCETIN
- Publication
Journal of Separation Science, 2023, Vol 46, Issue 13, p1
- ISSN
1615-9306
- Publication type
Article
- DOI
10.1002/jssc.202300041