We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cloning of the Rhizomucor miehei 3-hydroxy-3-methylglutaryl-coenzyme A reductase gene and its heterologous expression in Mucor circinelloides.
- Authors
Gyöngyi Lukács; Tamás Papp; Ferenc Somogyvári; Árpád Csernetics; Ildikó Nyilasi; Csaba Vágvölgyi
- Abstract
Abstract In this study, the gene hmgR encoding the 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase) was cloned and characterized in the zygomycete fungus Rhizomucor miehei. The hmgR gene comprises a total of 3,585 bp including the coding sequence of a 1,058 amino acids length putative protein and five introns (137, 83, 59, 60 and 69 bp in length) dispersed in the whole coding region. Southern hybridization analysis revealed that the gene is present only in one copy in the R. miehei genome. The isolated Rhizomucor gene was expressed in the related fungus, Mucor circinelloides. Transformants harbouring the Rhizomucor hmgR gene in an autoreplicative plasmid proved to be more tolerant to statins (e.g. lovastatin, simvastatin, and fluvastatin), the competitive inhibitors of the HMG-CoA reductase, than the original M. circinelloides strain. At the same time, heterologous expression of the Rhizomucor hmgR did not affect the carotenoid production of M. circinelloides.
- Subjects
MUCORACEAE; MUCORALES; ABSIDIA; ASEXUAL reproduction
- Publication
Antonie van Leeuwenhoek, 2009, Vol 95, Issue 1, p55
- ISSN
0003-6072
- Publication type
Article