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- Title
Zebrafish mutants with disrupted early T-cell and thymus development identified in early pressure screen.
- Authors
Trede, Nikolaus S.; Ota, Tatsuya; Kawasaki, Hirohide; Paw, Barry H.; Katz, Tammisty; Demarest, Bradley; Hutchinson, Sarah; Zhou, Yi; Hersey, Candace; Zapata, Agustin; Amemiya, Chris T.; Zon, Leonard I.
- Abstract
Generation of mature T lymphocytes requires an intact hematopoietic stem cell compartment and functional thymic epithelium. We used the zebrafish ( Danio rerio) to isolate mutations that affect the earliest steps in T lymphopoiesis and thymic organogenesis. Here we describe the results of a genetic screen in which gynogenetic diploid offspring from heterozygous females were analyzed by whole-mount in situ hybridization for the expression of rag-1. To assess immediately if a global defect in hematopoiesis resulted in the mutant phenotype, α-embryonic globin expression was simultaneously assayed for multilineage defects. In this report, we present the results obtained with this strategy and show representative mutant phenotypes affecting early steps in T-cell development and/or thymic epithelial cell development. We discuss the advantage of this strategy and the general usefulness of the zebrafish as a model system for vertebrate lymphopoiesis and thymic organogenesis. Developmental Dynamics 237:2575-2584, 2008. © 2008 Wiley-Liss, Inc.
- Publication
Developmental Dynamics, 2008, Vol 237, Issue 9, p2575
- ISSN
1058-8388
- Publication type
Article
- DOI
10.1002/dvdy.21683