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- Title
Methane production potential and methanogenic archaeal community structure in tropical irrigated Indian paddy soils.
- Authors
Dubey, Suresh; Singh, Alpana; Watanabe, Takeshi; Asakawa, Susumu; Singla, Ankit; Arai, Hironori; Inubushi, Kazuyuki
- Abstract
Soil characteristics regulate various belowground microbial processes including methanogenesis and, consequently, affect the structure and function of methanogenic archaeal communities due to change in soil type which in turn influences the CH production potential of soils. Thus, five different soil orders (Alfisol, Entisol, Inceptisol, Podzol and Vertisol) were studied to assess their CH production potential and also the methanogenic archaeal community structure in dryland irrigated Indian paddy soils. Soil incubation experiments revealed CH production to range from 178.4 to 431.2 μg CH g dws in all soil orders as: Vertisol<Inceptisol<Entisol<Podzol<Alfisol. The numbers of methanogens as quantified using real-time quantitative polymerase chain reaction (qPCR) targeting mcrA genes varied between 0.06 and 72.97 (×10 copies g dws) and were the highest in Vertisol soil and the least in Alfisol soil. PCR-denaturing gradient gel electrophoresis (DGGE)-based approach targeting 16S rRNA genes revealed diverse methanogenic archaeal communities across all soils. A total of 43 DGGE bands sequenced showed the closely related groups to Methanomicrobiaceae, Methanobacteriaceae, Methanocellales, Methanosarcinaceae, Methanosaetaceae and Crenarchaeota. The composition of methanogenic groups differed among all soils and only the Methanocellales group was common and dominant in all types of soils. The highest diversity of methanogens was found in Inceptisol and Vertisol soils. Methane production potential varied significantly in different soil orders with a positive relationship ( p < 0.05) with methanogens population size, permanganate oxidizable C (POXC) and CO production. The present study suggested that CH production potential of different soils depends on physicochemical properties, methanogenic archaeal community composition and the population size.
- Subjects
IRRIGATED soils; FARM manure in methane production; RICE soils; GEL electrophoresis; POLYMERASE chain reaction; SOIL classification
- Publication
Biology & Fertility of Soils, 2014, Vol 50, Issue 2, p369
- ISSN
0178-2762
- Publication type
Article
- DOI
10.1007/s00374-013-0858-7