We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Lactoferrin Expression by Bovine Ocular Surface Epithelia: A Primary Cell Culture Model to Study Lactoferrin Gene Promoter Activity.
- Authors
Maria Grazia MGS Santagati; Simona SL La Terra Mulè; Carla CA Amico; Matteo MP Pistone; Dario DR Rusciano; Vincenzo VE Enea
- Abstract
AbstractTear lactoferrin, mainly secreted by the lachrymal glands, exerts a protective effect on the ocular surface, and an abnormal decrease of its production may lead to an increased risk of infection and pathological alterations of ocular surface epithelia. In this study we analyzed whether corneal and conjunctival epithelia could be an additional source of tear lactoferrin, and whether conjunctival epithelial cells in culture could be a suitable model system to address regulation of lactoferrin gene expression. Real-time PCR and Western immunoblotting showed that in bovines lactoferrin is indeed produced by these epithelia, and that the human lactoferrin promoter can direct the expression of a CAT reporter gene, thus indicating that these cells are a true source of lactoferrin, and may be used in vitro to study the regulation of lactoferrin expression.Copyright © 2005 S. Karger AG, Basel
- Subjects
LACTOFERRIN; EPITHELIAL cells; GENE expression; GENETIC regulation; POLYMERASE chain reaction; WESTERN immunoblotting
- Publication
Ophthalmic Research, 2005, Vol 37, Issue 5, p270
- ISSN
0030-3747
- Publication type
Article
- DOI
10.1159/000087372