We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Peptide-Conjugated Quantum Dots Act as the Target Marker for Human Pancreatic Carcinoma Cells.
- Authors
Li, Shuang-ling; Yang, Jing; Lei, Xiao-fei; Zhang, Jian-na; Yang, Hong-li; Li, Kun; Xu, Chang-qing
- Abstract
Background/Aims: In the present study, we describe a novel and straightforward approach to produce a cyclic- arginine-glycine-aspartic (RGD)-peptide-conjugated quantum dot (QD) probe as an ideal target tumor biomarker. Due to its specific structure, the probe can be used for targeted imaging of pancreatic carcinoma cells. Methods: Pancreatic carcinoma cells were routinely cultured and marked with QD-RGD probe. The QD-RGD probe on the fluorescencelabeled cancer cell was observed by fluorescence microscopy and laser confocal microscopy. Cancer cell viability was detected by MTT assay after culturing with QD-RGD probe. Results: Fluorescence microscopy and laser confocal microscopy displayed that 10nmol/L QD-RGD probe was able to effectively mark pancreatic carcinoma cells. In comparison with organic dyes and fluorescent proteins, the quantum dot-RGD probe had unique optical and electronic properties. Conclusion: QD-RGD probe has a low cytotoxicity with an excellent optical property and biocompatibility. These findings support further evaluation of QD-RGD probes for the early detection of pancreatic cancer.
- Subjects
PANCREATIC cancer; CANCER cells; PEPTIDES; ARGININE; GLYCINE; QUANTUM dots
- Publication
Cellular Physiology & Biochemistry (Karger AG), 2016, Vol 38, Issue 3, p1121
- ISSN
1015-8987
- Publication type
Article
- DOI
10.1159/000443062