We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
一株鱼源致病性嗜水气单胞菌 XDMG 的全基因组测序 及比较基因组分析.
- Authors
郭少华; 毛会丽; 刘征权; 付美媛; 赵平原; 马文博; 李旭东; 关建义
- Abstract
The objectives of this study are to explore the mechanism of pathogenic Aeromonas hydrophila XDMG strain infecting fish, and to compare its genome structure, function and evolutionary relationship with the reference strain. The whole genome of A. hydrophila XDMG strain was sequenced, and the original data obtained by sequencing were assembled, spliced and annotated using Newbler, SeqMan, Glimmer and other software. Based on the constructing phylogenetic tree analysis from housekeeping genes and determining evolutionary relationships, the genomes of A. hydrophila were compared with those of four A. hydrophila strains in different regions. The complete genome sequence of A. hydrophila XDMG strain was 4.99 Mb in length and the GC content was 60.84%. A total of 4 935 coding genes were predicted, 4 137 genes with clear function were found, 99 tRNAs and 3 rRNAs were found; 4 061 genes had ortholog family classification, 3 228 genes were related to gene ontology, and 2 663 genes related to metabolic pathways; 101 tandem repeats and 14 gene islands were predicted in the genome of A. hydrophila XDMG strain, with genes related to three QS systems: AI 1, AI 2, and AI 3. Through genome comparative analysis, A. hydrophila XDMG was closely related to domestic A. hydrophila J-1 strain and US-derived A. hydrophila AL0971 strain; it was more conserved with US-derived A. hydrophila AL0971 in the comparison of virulence genes, outer membrane proteins, and O-antigen gene clusters. Comprehensive genome annotation and comparative analysis of the genome of A. hydrophila XDMG strain provide basic data for the study of functional genomics and genetic engineering vaccines of A. hydrophila.
- Subjects
WHOLE genome sequencing; GENETIC engineering; TANDEM repeats; AEROMONAS hydrophila; DNA vaccines; GENETIC software; TRANSFER RNA; SHORT tandem repeat analysis
- Publication
Biotechnology Bulletin, 2023, Vol 39, Issue 8, p291
- ISSN
1002-5464
- Publication type
Article
- DOI
10.13560/j.cnki.biotech.bull.1985.2022-1507