We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Estrogen-Like Response to p-Nonylphenol in Human First Trimester Placenta and BeWo Choriocarcinoma Cells.
- Authors
Bechi, Nicoletta; Ietta, Francesca; Romagnoli, Roberta; Focardi, Silvano; Corsi, Ilaria; Buffi, Carlo; Paulesu, Luana
- Abstract
p-Nonylphenol (p-NP) is a metabolite of alkylphenol ethoxylates used as surfactants in the manufacturing industry. Although it is reported to have estrogenic activity and to be transferred from the mother to the embryo, no data are available on its effects on the development of the human placenta. In the present study, we investigated estrogen receptors' (ERs) expression in the first trimester human placenta. Using an in vitro model of chorionic villous explants, we then compared the effects of p-NP and 17β-estradiol (17β-E2). Finally, a trophoblast-derived choriocarcinoma cell line, BeWo, was used as a model of trophoblast cell differentiation. Our results showed that the first trimester placenta expresses three ER-α isoforms of 67, 46, and 39 kDa and one ER-β isoform of 55 kDa. Immunohistochemistry revealed the expression of ER-α in the villous cytotrophoblast, whereas ER-β was mainly expressed by the syncytiotrophoblast. Treatment of explant cultures with p-NP (10−9M) and 17β-E2 (10−9M) significantly increased β-hCG secretion and cell apoptosis but did not modify ER expression. After 72 h of exposure, hormone release was significantly higher in p-NP– than 17β-E2–treated explant cultures. By this time, cleavage of caspase-3 was evident in cultures treated with 17β-E2 and p-NP. In BeWo cells, a caspase-3 band of 20–16 kDa was evident after 1 h of treatment with p-NP and after 24 h of treatment with 17β-E2 or forskolin. These findings suggest that the human trophoblast may be highly responsive to p-NP and raise concern about maternal exposure in early gestation.
- Subjects
PHENOL; ESTROGEN; PLACENTA; TROPHOBLAST; APOPTOSIS
- Publication
Toxicological Sciences, 2006, Vol 93, Issue 1, p75
- ISSN
1096-6080
- Publication type
Article
- DOI
10.1093/toxsci/kfl043