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- Title
Inhibitory Effects of Curcumin and 2-Methoxyestradiaul on Anaphase Promoting Complex in Hela Cancer Cells.
- Authors
Rahimi, Hamzeh; Foroumadi, Alireza; Shokrgozar, Mohammad Ali; Mahdian, Reza; PharmD, Armin Madadkar-Sobhani; Karimipoor, Morteza
- Abstract
Background: Anaphase promoting complex (APC) plays a critical role in cell division and mitotic exit. This protein complex may have a pivotal role in the cell cycle control affecting pathological conditions such as cancer. APC is recommended as the target of many anti-cancer agents due to its importance in cancer pathogenesis. This study aimed to evaluate the inhibitory effects of curcumin and 2-methoxyestradiaul on APC. Methods: Hela cells were treated with various concentrations of curcumin and 2-methoxyestradiaul,and their cytotoxic effects were investigated after 24,48,72 and 96 hours by MTT assay. Expression of securin,cyclin B and the APC substrates were investigated using immune-blotting. Finally,cell cycle analysis was performed using DRAQ5 staining and flow cytometry. Findings: Respectively,20 and 30 percent of cell-death after 24-hour treatment with curcumin and 2- methoxyestradiaul was seen. Also,securin and cyclin levels were raised after 24 hours of treatment. Furthermore,the levels of the substrates (securin and cyclin B) increased 48 hours after treatment. Results of fluorescence-activated cell sorting (FACS) analysis showed that treated cells were arrested in G2 phase of cell cycle which revealed cell arrest in G2 phase of cell cycle. 2-methoxyestradiaul showed a better APC inhibition effect than curcumin (P = 0.02). Conclusion: Our results revealed that APC is a suitable target for cancer treatment and curcumin and 2-methoxyestradiaul have inhibitory effects on the activity of this complex.
- Subjects
ANAPHASE; CELL cycle; CELL death; CANCER treatment; CELL proliferation; CURCUMIN; FLOW cytometry
- Publication
Journal of Isfahan Medical School, 2013, Vol 31, Issue 228, p255
- ISSN
1027-7595
- Publication type
Article