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- Title
Simultaneous fermentative chitinase and β-1,3 glucanase production from Streptomyces philanthi RM-1-1-38 and their antifungal activity against rice sheath blight disease.
- Authors
BOUKAEW, SAWAI; PETLAMUL, WANIDA; SUYOTHA, WASANA; PRASERTSAN, POONSUK
- Abstract
Chitinase and β-1,3 glucanase are enzymes that play important roles in the biocontrol of fungal plant pathogens.The effects of environmental conditions and culture medium composition on simultaneous chitinase and β-1,3 glucanase production from Streptomyces philanthi RM-1-138 were investigated using a conventional (one-factorat- a-time) method and a response surface methodology (RSM), respectively. The optimum cultivation conditions were at pH 7.5 and a temperature of 30EC. The optimized medium (4.88 g @ l-1 chitin, 6.27 g @ l-1glucose, and 17.05 g @ l-1>malt extract) exhibited 53- and 80-fold increase in the activity of chitinase (0.53 U @ ml-1) and β-1,3 glucanase (8.79 U @ ml-1), respectively, compared to the original medium. The culture filtrate from the original Chitinase and the optimized medium were partially purified and tested (by agar-well diffusion assay) for their antifungal activities against Rhizoctonia solani PTRRC-9 compared to the chemical fungicides carbendazim®and propiconazole ®(100 μg @ ml-1). The partially purified enzymes from the optimized medium exhibited 4.0-fold stronger antifungal activities against R. solani PTRRC-9 compared to that from the original medium and equal to that of the chemical fungicide propiconazole®but slightly lower than that of carbendazim®.
- Subjects
RICE sheath blight treatment; CHITINASE; GLUCANASES; ANTIFUNGAL agents; RESPONSE surfaces (Statistics); FUNGAL diseases of plants
- Publication
Biotechnologia, 2016, Vol 97, Issue 4, p271
- ISSN
0860-7796
- Publication type
Article
- DOI
10.5114/bta.2016.64544