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- Title
Staphylococcus aureus as source of catheter-related bloodstream infection evaluated by PFGE and rep-PCR typing in a Brazilian hospital.
- Authors
SADOYAMA, GERALDO; DOS SANTOS, KÁTIA REGINA NETTO; BRILHANTE, ANIKE PEREIRA; GONTIJO FILHO, PAULO PINTO
- Abstract
Staphylococci are a common cause of catheter-related bloodstream infection (CR-BSI), and epidemiological typing is an important tool for effective infection control. This study evaluated by PFGE and rep-PCR whether Staphylococcus aureus strains isolated from skin and catheter tips were related to specimens isolated from blood. A prospective observational study, carried out in a clinical surgical ward at a Brazilian hospital between September 2000 and November 2002, investigated non-tunneled central venous catheters from 179 patients. S. aureus isolates were mainly obtained from blood (41.4%), while coagulase-negative staphylococci strains were more often isolated from the skin at the catheter insertion site (49.7%) and from the catheter tip (57.5%). Among the 21 strains isolated from 9 patients at 2 or 3 sites simultaneously, 9 were methicillin-resistant S. aureus (MRSA) and 12 were methicillin-susceptible S. aureus (MSSA). Seven patients harbored the same S. aureus strain isolated from the skin, blood and/or catheter tip cultures. MRSA isolates belonged to one PFGE pattern (type A- subtypes A1, A2 and A3), and to two rep-PCR patterns (a and b). MSSA isolates were distinguished in five PFGE (B to F) and in three rep-PCR (c, d and e) patterns. Both PFGE and rep-PCR methods indicated that the skin at the catheter insertion site was the origin of CR-BSI caused by S. aureus.
- Subjects
STAPHYLOCOCCUS aureus infections; STAPHYLOCOCCAL diseases; PENICILLIN; ANTIBACTERIAL agents; METHICILLIN resistance; DRUG resistance in microorganisms
- Publication
APMIS, 2008, Vol 116, Issue 11, p953
- ISSN
0903-4641
- Publication type
Article
- DOI
10.1111/j.1600-0463.2008.01053.x