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- Title
Evaluation of CAR-T Cells' Cytotoxicity against Modified Solid Tumor Cell Lines.
- Authors
Valiullina, Aigul Kh.; Zmievskaya, Ekaterina A.; Ganeeva, Irina A.; Zhuravleva, Margarita N.; Garanina, Ekaterina E.; Rizvanov, Albert A.; Petukhov, Alexey V.; Bulatov, Emil R.
- Abstract
In recent years, adoptive cell therapy has gained a new perspective of application due to the development of technologies and the successful clinical use of CAR-T cells for the treatment of patients with malignant B-cell neoplasms. However, the efficacy of CAR-T therapy against solid tumor remains a major scientific and clinical challenge. In this work, we evaluated the cytotoxicity of 2nd generation CAR-T cells against modified solid tumors cell lines—lung adenocarcinoma cell line H522, prostate carcinoma PC-3M, breast carcinoma MDA-MB-231, and epidermoid carcinoma A431 cell lines transduced with lentiviruses encoding red fluorescent protein Katushka2S and the CD19 antigen. A correlation was demonstrated between an increase in the secretion of proinflammatory cytokines and a decrease in the confluence of tumor cells' monolayer. The proposed approach can potentially be applied to preliminarily assess CAR-T cell efficacy for the treatment of solid tumors and estimate the risks of developing cytokine release syndrome.
- Subjects
3M Co.; CELL lines; CD19 antigen; CYTOKINE release syndrome; SQUAMOUS cell carcinoma; FLUORESCENT proteins
- Publication
Biomedicines, 2023, Vol 11, Issue 2, p626
- ISSN
2227-9059
- Publication type
Article
- DOI
10.3390/biomedicines11020626