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- Title
补肾活血方对去卵巢大鼠子宫ER及Cyclin B表达影响的实验研究.
- Authors
唐雨晴; 张东伟; 孙雪娇; 郭鱼波; 王丽丽; 马如风; 李城; 刘祎; 李彧
- Abstract
To explore the effects of Bushen Huoxue (nourishing kidney and activating blood) Formula on estrogen receptor (ER) and Cyclin B in ovariectomized rats ' uteri,so as to study the mechanism of Bushen Huoxue Formula (TCM) on perimenopausal syndrome. Methods Total 54 female SD rate were randomly divided into sham group, model group, estradiol group, TCM group, TCM + estradiol group,TCM + sham group {n-9 each). Rat model of perimenopausal syndrome was induced by bilateral ovariectomy. After 40 days' intragastrical administration, all the rats were sacrificed. Rats' uteri were dissected and weighed. The serum contents of estrogen. (E2),luteinizing hormone (LH) and follicle stimulating hormone (FSH) were detected by using ELISA; the endometrial thickness and pathological changes were measured using HE staining ; the distribution, and the expression of ERα and ERβ in endometrial tissue were observed by immunohistochemical method ; the protein expression of Cyclin B was detected by Western Blot. Results Compared with the model group,the serum content of LH and FSH decreased significantly (P < 0. 05, P < 0. 01) and that of E2 increased in TCM group (P < 0. 05) ; while the index of uterus, endometrial thickness and the protein expressions of ERα,ERβ and Cyclin B increased remarkably (P < 0. 05). The estrogen-like effects of Bushen Huoxue Formula was weaker than estrogen. And each indicator of TCM + sham group showed no difference with that of sham group(P >0, 05). Conclusion The estrogen-like effects of Bushen Huoxue Formula were proved to be weaker than that of E2, with no significant impact on uteri tissue of healthy rats.
- Publication
Journal of Beijing University of Traditional Chinese Medicine, 2016, Vol 39, Issue 4, p281
- ISSN
1006-2157
- Publication type
Article
- DOI
10.3969/j.issn.1006-2157.2016.04.004