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- Title
An effective microinjection method for genome editing of marine aquaculture fish: tiger pufferfish Takifugu rubripes and red sea bream Pagrus major.
- Authors
Kishimoto, Kenta; Washio, Youhei; Murakami, Yu; Katayama, Takashi; Kuroyanagi, Miwa; Kato, Keitaro; Yoshiura, Yasutoshi; Kinoshita, Masato
- Abstract
Genome editing technology is becoming increasingly accepted as a way to improve traits in marine fish aquaculture. In fish, microinjection is a major method for introducing RNA or protein into eggs for genome editing; however, this method has not yet been established in aquaculture fish. We successfully established microinjection methods achieving high survival rates for tiger pufferfish and red sea bream by optimizing the following three parameters: (1) the soaking solution of fertilized eggs during microinjection, (2) the elapsed time from in vitro fertilization to microinjection, (3) the elapsed time from stripping to microinjection. In tiger pufferfish, Iwamatsu solution or diluted sea water is effective as the soaking solution. In vitro fertilization can be performed at intervals of 15 min from fertilization until 2.5 h after stripping. Similarly, in red sea bream, Leibovitz's L-15 medium or Iwamatsu solution is effective as the soaking solution and in vitro fertilization can be performed at intervals of 10 min from fertilization until 2.5 h after stripping. We anticipate our findings will contribute to effectively establish genome edited aquaculture breeds.
- Subjects
MICROINJECTION (Cytology); RNA; PROTEINS; GENOME editing; CRISPRS; OSTEICHTHYES; AQUACULTURE; FISH fertility
- Publication
Fisheries Science, 2019, Vol 85, Issue 1, p217
- ISSN
0919-9268
- Publication type
Article
- DOI
10.1007/s12562-018-1277-3