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- Title
Effect of storage conditions on the clonogenic potential of hematopoietic stem cells for transplantation.
- Authors
Matuszak, Paula; Kozłowska-Skrzypczak, Maria; Dziatkiewicz, Paulina; Bembnista, Ewa; Kubiak, Agnieszka; Majewska, Ewa; Kaźmierska, Katarzyna; Gil, Lidia
- Abstract
Hematopoietic stem cell (HSC) transplantation is an important element of the treatment of hematooncologic diseases. It is essential that the transplantation material be of the highest quality. We analyzed the effect of storage time and temperature of hematopoietic cell preparations on the clonogenic potential of hematopoietic cells. We analyzed 31 autologous HSC samples. The clonal growth of granulocyte-monocyte colony forming unit (CFU-GM) precursors was assessed on the day of apheresis and after 24 h and 48 h of storage at 4°C and 20°C before freezing the samples. Clonogenic potential was evaluated byclonogenic assay in vitro after 7-10 days incubation (37°C, 5% CO2, 100% humidity). The material for the study was obtained at the Department of Hematology and Bone Marrow Transplantation, Poznań University of Medical Sciences. The number of CFU-GM precursors stored at 4°C, assessed before freezing was 419 CFU-GM/105 mononuclear cells (MNC) on the day of apheresis, 318 CFU-GM/105 MNC after 24 h storage and 260 CFU-GM/105 MNC after 48 h storage, respectively (p = 0.00081). In the material stored at 20°C evaluated before freezing, the number of precursors was 83 CFU-GM/105 after 24 h storage, while no precursors were found after 48 h storage (p <0.000001). On the basis of the obtained results of the analysis of the storage conditions of the transplantation material, it can be concluded that the clonogenic potential of CFU-GM evaluated in the material stored for 24 and 48 h at 4°C allows to qualify the material for transplantation. Storage of the transplanted material for 24 h and 48 h at 20°C causes a significant decrease in the clonogenic potential, disqualifying the product from the transplantation procedure. Due to a significant decrease of the clonogenic potential of HSC at room temperature, all preparative steps should be performed in the shortest possible time.
- Subjects
HEMATOPOIETIC stem cell transplantation; BONE marrow transplantation; HEMATOPOIETIC stem cells
- Publication
Acta Haematologica Polonica, 2022, Vol 53, Issue A, p79
- ISSN
0001-5814
- Publication type
Article