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- Title
The molecular characterization of two barley proteins establishes the novel PR-17 family of pathogenesis-related proteins.
- Authors
Christensen, Anders B; Cho, Baik HO; Næsby, Michael; Gregersen, Per L; Brandt, Jakob; Madriz-Ordeñana, Kenneth; Collinge, David B; Thordal-Christensen, Hans
- Abstract
Summary Two barley (Hordeum vulgare L.) cDNA clones (pBH6-12 and pBH6-17) were isolated from a cDNA library prepared from leaves 6 h after inoculation with Blumeria graminis f.sp. hordei (Bgh ). The two transcripts accumulate strongly in response to Bgh , peaking around 6, 15–24 and 48–96 h after inoculation, concomitant with fungal penetration attempts, hypersensitive response and fungal growth. The encoded proteins, Hv PR-17a and Hv PR-17b, belong to a new family of plant pathogenesis-related proteins, designated ‘PR-17’. The family also include NtPRp27 from tobacco (Okushima et al ., 2000, Plant Mol. Biol. 42 , 479–488) and WCI-5 from wheat (Görlach et al ., 1996, Plant Cell 8 , 629–643), responsive to viral and fungal infection, respectively. Antisera were raised to Hv PR-17a and Hv PR-17b, and the proteins exhibit apparent molecular weights of 26 and 24 kDa, respectively. They accumulate in the mesophyll apoplast following Bgh -inoculation, as well as in the leaf epidermis, the only tissue to be invaded by the fungus. Several homologous plant proteins exist, and a highly conserved part of the members of this new protein family show similarity to the active site and to the peptide-binding groove of the exopeptidase ‘aminopeptidase N’ from eukaryotes and the endopeptidase ‘thermolysin’ from bacteria.
- Subjects
BARLEY; PLANT proteins; PLANT chromosomes; MOLECULAR genetics
- Publication
Molecular Plant Pathology, 2002, Vol 3, Issue 3, p135
- ISSN
1464-6722
- Publication type
Article
- DOI
10.1046/j.1364-3703.2002.00105.x