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- Title
Selective condensation of DNA by aminoglycoside antibiotics.
- Authors
Kopaczynska, M.; Schulz, A.; Fraczkowska, K.; Kraszewski, S.; Podbielska, H.; Fuhrhop, J.
- Abstract
The condensing effect of aminoglycoside antibiotics on the structure of double-stranded DNA was examined. The selective condensation of DNA by small molecules is an interesting approach in biotechnology. Here, we present the interaction between calf thymus DNA and three types of antibiotic molecules: tobramycin, kanamycin, and neomycin. Several techniques were applied to study this effect. Atomic force microscopy, transmission electron microscopy images, and nuclear magnetic resonance spectra showed that the interaction of tobramycin with double-stranded DNA caused the rod, toroid, and sphere formation and very strong condensation of DNA strands, which was not observed in the case of other aminoglycosides used in the experiment. Studies on the mechanisms by which small molecules interact with DNA are important in understanding their functioning in cells, in designing new and efficient drugs, or in minimizing their adverse side effects. Specific interactions between tobramycin and DNA double helix was modeled using molecular dynamics simulations. Simulation study shows the aminoglycoside specificity to bend DNA double helix, shedding light on the origins of toroid formation. This phenomenon may lighten the ototoxicity or nephrotoxicity issues, but also other adverse reactions of aminoglycoside antibiotics in the human body.
- Subjects
CONDENSATION; DNA analysis; AMINOGLYCOSIDES; SMALL molecules; BIOTECHNOLOGY research
- Publication
European Biophysics Journal, 2016, Vol 45, Issue 4, p287
- ISSN
0175-7571
- Publication type
Article
- DOI
10.1007/s00249-015-1095-9